Therapeutic effect of hydroalcoholic extract of Moringa oleifera and sulfurphane on melanoma caused by B16f10 cell line in mouse model C57bl/6

Document Type : Original Article


1 Amiran Oghyanoos Biotechnology Company, Tehran, Iran

2 Skin Diseases and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Iran Food and Drug Administration, Tehran, Iran

4 Department of Environmental Engineering, School of Environmental, University of Tehran, Iran

5 Department of Environmental Science, University of Agricultural Sciences and Natural Resources, Gorgan, Iran


Background: Moringa oleifera, an herbal medicine, has been found to possess anti-tumor effects against various cancers.
Objectives: The aim of this study was to investigate the anti-inflammatory and antioxidant properties of Moringa oleifera plant extract on metastasis.
Methods: Initially, the presence of phenolic compounds in Moringa oleifera was confirmed through HPLC and spectrophotometer methods. Following that, the anticancer effects of the phenolic chemicals sulforaphane and Moringa oleifera on B16F10 cancer cells were investigated. Following this, melanoma tumors were induced in C57BL6 mice using the B16F10 cell line, and the mice were treated with the extract. The extract was identified to contain quercetin, gallic acid, and caffeic acid.
Results: The rate of cell death observed in the sulforaphane-exposed cell group was lower compared to the group treated with Moringa oleifera. Furthermore, tumor volume decreased significantly after one and two weeks of therapy with all three doses of Moringa extract or phenolic chemicals (P<0.001). Notably, the tumor volume decreased the most with the 0.02 g dose compared to the other two doses (P <0.001).
Conclusion: This study demonstrates the positive effect of antioxidants present in Moringa oleifera extract on reducing the viability of cancer cells and tumor volume in mice. Further research is recommended to establish the potential of Moringa oleifera as an effective anticancer drug with greater certainty. Additionally, it is advisable to conduct studies on human subjects as well.


Main Subjects

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